Design, Synthesis, and Characterization of N-Oxide-Containing Heterocycles with in Vivo Sterilizing Antitubercular Activity

نویسندگان

  • Guilherme Felipe Dos Santos Fernandes
  • Paula Carolina de Souza
  • Elsa Moreno-Viguri
  • Mery Santivañez-Veliz
  • Rocio Paucar
  • Silvia Pérez-Silanes
  • Konstantin Chegaev
  • Stefano Guglielmo
  • Loretta Lazzarato
  • Roberta Fruttero
  • Chung Man Chin
  • Patricia Bento da Silva
  • Marlus Chorilli
  • Mariana Cristina Solcia
  • Camila Maríngolo Ribeiro
  • Caio Sander Paiva Silva
  • Leonardo Biancolino Marino
  • Priscila Longhin Bosquesi
  • Debbie M Hunt
  • Luiz Pedro S de Carvalho
  • Carlos Alberto de Souza Costa
  • Sang Hyun Cho
  • Yuehong Wang
  • Scott Gary Franzblau
  • Fernando Rogério Pavan
  • Jean Leandro Dos Santos
چکیده

Tuberculosis, caused by Mycobacterium tuberculosis (Mtb), is the infectious disease responsible for the highest number of deaths worldwide. Herein, 22 new N-oxide-containing compounds were synthesized followed by in vitro and in vivo evaluation of their antitubercular potential against Mtb. Compound 8 was found to be the most promising compound, with MIC90 values of 1.10 and 6.62 μM against active and nonreplicating Mtb, respectively. Additionally, we carried out in vivo experiments to confirm the safety and efficacy of compound 8; the compound was found to be orally bioavailable and highly effective, leading to a reduction of Mtb to undetectable levels in a mouse model of infection. Microarray-based initial studies on the mechanism of action suggest that compound 8 blocks translation. Altogether, these results indicate that benzofuroxan derivative 8 is a promising lead compound for the development of a novel chemical class of antitubercular drugs.

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عنوان ژورنال:

دوره 60  شماره 

صفحات  -

تاریخ انتشار 2017